Modelling the lifespan of human lymphocyte subsets

maximum likelihood estimation by embedded simulation by A. S. Hurn

Publisher: Glasgow University Department of Political Economy in Glasgow

Written in English
Published: Downloads: 320
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Edition Notes

StatementA.S.Hurn andC.A.Michie.
SeriesEconomics discussion paper series / University of Glasgow, Department of Political Economy -- no.9403, Economics discussion paper (University of Glasgow, Department of Political Economy) -- no.9403.
ContributionsMichie, C.A.
ID Numbers
Open LibraryOL19559605M

  In vivo models of human immunity in human tumor-bearing mice are frequently used to study mechanisms of targeted immunotherapies, safety, and efficacy. In . Books; JCB Journal of Cell Biology; is relatively constant throughout life. According to this model, the telomere loss rates in granulocytes and naive T cells are expected to be equal, even if these cells and their immediate precursors are dividing at different rates. Lifespan of human lymphocyte subsets defined by CD45 isoforms. Nature. lymphocyte subsets () Filter by: Remove filter: t-lymphocyte subsets - drug effects () Filter by: Remove filter: mice, transgenic () Filter by: Remove filter: t-lymphocytes, regulatory - immunology () Filter by: Remove filter: analysis () T helper 17 (T H 17) cells are key players in multiple sclerosis (MS), and studies in animal models demonstrated that effector T H 17 cells that trigger brain autoimmunity originate in the intestine. We validate in humans the crucial role of the intestinal environment in promoting T H 17 cell expansion in MS patients. We found that increased frequency of T H 17 cells correlates with high.

Chronic hepatitis B virus (HBV) infection is one of the high-risk factors for human HCC. Despite the integration of virus DNA and the oncoprotein HBx, chronic necroinflammation and hepatocellular regeneration account for hepatocarcinogenesis. As a non-cytopathic virus, HBV is extensively recognized . Naive T lymphocytes travel to T-cell areas of secondary lymphoid organs in search of antigen presented by dendritic cells1,2. Once activated, they proliferate vigorously, generating effector cells.   Schaberg T, Theilacker C, Nitschke OT, Lode H. Lymphocyte subsets in peripheral blood and smoking habits. Lung Schultz C, Reiss I, Bucsky P, Gopel W, Gembruch U, Ziesenitz S, et al. Maturational changes of lymphocyte surface antigens in human blood: comparison between fetuses, neonates and adults. Biol Neonate 78(2) Systemic lupus erythematosus (SLE) is a potentially life-threatening autoimmune disease characterized by altered balance of activity between effector and regulatory CD4(+) T cells. The homeostasis of CD4(+) T cell subsets is regulated by interleukin (IL)-2, and reduced production of IL-2 by T cells .

As of April , the lineage relationship between effector and memory T cells is unclear. Two competing models exist. One is called the On-Off-On model. When naive T cells are activated by T cell receptor (TCR) binding to antigen and its downstream signaling pathway, they actively proliferate and form a large clone of effector cells. Effector cells undergo active cytokine secretion and other. Combination of RT with IL-2 could be an alternative. But IL-2 has a short half-life, and, by binding to its high-affinity receptor, it strongly stimulates immunosuppressive CD4+ Tregs and seems to promote potentially life-threatening vascular leakage. B cells, also known as B lymphocytes, are a type of white blood cell of the lymphocyte subtype. They function in the humoral immunity component of the adaptive immune system by secreting antibodies. Additionally, B cells present antigens (they are also classified as professional antigen-presenting cells (APCs)) and secrete cytokines. In mammals, B cells mature in the bone marrow, which is at. A massive decrease in blood lymphocyte count was prominent in the mouse model of ZEBOV infection, with NK and CD8 + cells the first lymphocyte subsets to be depleted. NK cells fell to 38% of their normal numbers on day 2 of infection and to 20% on day 3 (figure 4F).

Modelling the lifespan of human lymphocyte subsets by A. S. Hurn Download PDF EPUB FB2

E~E~ER Modeling the Lifespan of Human T Lymphocyte Subsets A. HURN Brasenose College, The University of Oxford, Oxford, England K. LINDSAY Department of Mathematics, University Gardens, The University of Glasgow, Glasgow, Scotland AND C.

MICHIE Department of Paediatrics and Neonatal Medicine, The Royal Postgraduate Medical School, London, Cited by: 2. Modeling the lifespan of human T lymphocyte subsets.

(PMID) Abstract This phenomenon is modeled by a mechanism qualitatively similar to Demoivre's law of human mortality. both lymphocyte subsets undergo decay. Model parameters and their standard errors are estimated by maximizing a likelihood function constructed by Kalman Cited by: 2.

Using the observation that T lymphocyte expression of isoforms of CD45 corresponds with their ability to respond to recall antigens, we have investigated the lifespan of both CD45RO (the subset Cited by: Michie CA, Mclean A, Alcock C, Beverley PC () Lifespan of human lymphocyte subsets defined by CD45 isoforms.

Nature – PubMed CrossRef Google Scholar Miyasaka M, Trnka Z () Lymphocyte migration and differentiation in a large animal odel: the by: The modelling results indicate that it is unlikely that long-lived human recirculating 'memory' PBTL are homogeneous Modelling the lifespan of human lymphocyte subsets book respect to lifespan, but rather that two major subpopulations, T1 and T2, are involved having mean lifespans (and approximately 95% confidence limits) estimated to be approximately () and approximately () year, by: Fig.

S1 quantifies human blood monocyte subset membrane marker expression and cell cycle analysis. Fig. S2 shows modeling curves generated with and without a delay between intermediate and nonclassical monocytes.

Table S1 shows model data fit with and without a delay. Table S2 shows lifespans, proliferation rates, and delays for the model with. Michie C, McLean A, Alcock C, Beverley P () Lifespan of human lymphocyte subsets defined by CD45 isoforms. Nature – PubMed CrossRef Google Scholar 4.

A recent study by Rossi et al. describes the miRNA-ome of most human lymphocyte subsets. miRa-5p, which we found in our study specifically expressed in T cells, was also found to be expressed by CD4 and CD8 cells, in the study of Rossi et al.

In concordance with their results we also found expression of miR in all B, T and NK cells. Gossel et al. used two experimental approaches to study the dynamics of memory T cells in mice.

(A) To quantify the influx of naive T cells into the pool of existing memory T cells, they used the drug busulfan to selectively kill hematopoietic stem cells (HSC) in the bone marrow of CD+ mice (red arrow), and then added new cells derived from bone marrow of CD+ mice (green arrow).

Memory B cells have several unique features including long lifespan, high sensitivity to low doses of antigen, quick and robust proliferation, and rapid differentiation into plasma cells that produce high-affinity antibodies during the secondary response.

CD27 is a marker for human memory B cells (Klein, Rajewsky, & Kuppers, ). Lymphocyte subsets and their level of variability also differ in subjects with diseases such as SLE (6, ). Therapy with corticosteroids and other immunomodulatory agents can have profound effects on lymphocyte subset distributions as well (2, ), further complicating longitudinal data interpretation in the setting of disease.

In the adult mouse, three major subsets of mature B-lymphocytes are recognized: B-1, marginal zone (MZ), and follicular (FO) B cells. These B lymphocytes are the products of B-cell precursors that follow developmental programs that require passage through successive checkpoints to produce appropriately matured B-cell clonotypes.

In book: Systems and Synthetic Immunology (pp) the role of various defined T lymphocyte subsets (Th cells comprising Th1, Th2, Th9, Th17, Th22, regulatory T cells, follicular helper T. The clonal half-life is the time for a T cell clone to halve in size whilst the overall population remains at steady state (i.e.

without halting proliferation) and likely relates to the longevity of a T cell clone (for zero influx the clonal half-life is infinite). Models describing an upstream/precursor population and a downstream/target cell.

Abstract. Infection of mice with the Duplan-Latarjet strain of RadLV, also called RadLV-Rs, induces a syndrome characterised by polyclonal proliferation of B- and CD4+ T-cells and the simultaneous appearance of an in vitro refractoriness to mitogen stimulation involving both lymphocyte subsets.

This syndrome, initially observed by Mistry and Duplan l and described later as MAIDS (for Murine. Lymphocytes Subsets Reference Values in Childhood F. Tosato,1* G. Bucciol,2 G. Pantano,1 M. Putti,2 M.C. Sanzari,1 G. Basso,2 M. Plebani1 Abstract Immunophenotyping of blood lymphocyte subsets and activation markers is a basic tool in the diagnostic process of primary immunodeficiency diseases, its use becoming.

The immune system plays a critical role in protecting hosts from the invasion of organisms. CD4 T cells, as a key component of the immune system, are central in orchestrating adaptive immune responses.

After decades of investigation, five major CD4 T helper cell (Th) subsets have been identified: Th1, Th2, Th17, Treg (T regulatory), and Tfh (follicular T helper) cells. Throughout life, T cells coordinate multiple aspects of adaptive immunity, including responses to pathogens, allergens, and tumors.

In mouse models, the role of T cells is studied in the context of a specific type of pathogen, antigen, or disease condition over a limited time frame, whereas in humans, T cells control multiple insults simultaneously throughout the body and maintain immune.

Ruco LP, Stoppacciaro A, Valtieri M, Uccini S, Vitolo D, Baroni CD () T-lymphocyte subsets in human lymph nodes: relative increase of OKT-8 + cells in neoplastic and reactive B-cell proliferation. Clin Immunol Immunopathol – Google Scholar. The four main human B lymphocyte subsets were isolated from blood of healthy adults and analyzed for their replication history.

Naive CD5 − B lymphocytes in adult peripheral blood were found to have a replication history of (Fig. 6 B), which is almost similar to the total bulk of naive mature B lymphocytes in tonsils from children. Buckton KE, Brown WM, Smith PG.

Lymphocyte survival in men treated with x-rays for ankylosing spondylitis. Nature. Apr 29; ()– Michie CA, McLean A, Alcock C, Beverley PC.

Lifespan of human lymphocyte subsets defined by CD45 isoforms. Nature. Nov 19; ()– The pattern of appearance of labeled lymphocytes was consistent with the kinetics of transitional cells that function as their precursors.

Thus, in the bone marrow of the guinea pig the majority of lymphocytes have a short lifespan and a rapid turnover, whereas about 14% turn over more slowly and 7% have a life-span exceeding 4 wk.

A lymphocyte is a type of white blood cell in the vertebrate immune system. Lymphocytes include natural killer cells (which function in cell-mediated, cytotoxic innate immunity), T cells (for cell-mediated, cytotoxic adaptive immunity), and B cells (for humoral, antibody-driven adaptive immunity).

They are the main type of cell found in lymph, which prompted the name "lymphocyte". Multiple CD4 + and CD8 + T-cell subsets were measured by flow cytometry, and prevalent diabetes cases were adjudicated by 2 physicians for PWH and HIV-negative participants in the Veterans Aging Cohort Study.

Multivariable logistic regression models evaluated the association of T-cell subsets and diabetes stratified by HIV status, adjusted for cytomegalovirus serostatus and traditional risk.

Differentiation of immature T and B cells in the primary lymphoid organs gives rise to a pool of long-lived lymphocytes that recirculate through the secondary lymphoid tissues. On the basis of their surface markers, T and B cells comprise a mixture of naive and memory cells with differing life-spans.

Immunization (and vaccination) causes naive lymphocytes to proliferate and differentiate into. Cities. T lymphocyte subsets were analyzed to get the absolute counts and percentages of the T lymphocyte subsets to establish the normal reference range of T lymphocyte subsets of health adult in the Inner Mongolia Region.

The details were as follows: (1) Preparation of samples and reagents for detection; (2) 20 μl anti-CD3/CD4/CD45 antibody was. Two divergent models have been proposed to explain the existence of the main subsets of B lymphocytes: the B-1 and B-2 (FO and MZ) cell populations.

The first model proposes the existence of two distinct B-lineage-specific progenitors that give rise to B-1 and B-2 cells, respectively. This is referred to as the dual-lineage model.

As shown in Figure 1, the total lymphocyte and T lymphocyte absolute counts are very high in the first months of life and decrease with age by two thirds, but the relative frequency of T cells remains stable, with a median ranging from 67% to 73%.

Also CD4+ and CD8+ T cells show a certain stability with age, with median limits of 38–53% for. Subsets of CD4+ and CD8+ peripheral T-cells, platelets and monocytes.

The CD60 family are oligosaccharides present on gangliosides and are involved in co-stimulation of T-cells. CD60b: UM4D4, 9-O-acetyl-GD3: Subsets of CD4+ and CD8+ peripheral T-cells, platelets and monocytes. In the adult mouse, three major subsets of mature B-lymphocytes are recognized: B-1, marginal zone (MZ), and follicular (FO) B cells.

These B lymphocytes are the products of B-cell precursors that. The co-ordinated recruitment of monocyte subpopulations, neutrophils and regulatory T-cells (Tregs) during the early stages of human acute lung inflammation remains poorly understood.

We therefore performed a detailed characterisation of these lineages in the blood and lungs in a model of human acute lung inflammation. Healthy volunteers inhaled lipopolysaccharide (LPS) or saline (n=6 for each.

The median time to maximal concentration ranged from to h, and ponesimod was eliminated with a mean half-life varying between and h. Food had a minimal effect on ponesimod pharmacokinetics.

Doses of ≥8 mg reduced total lymphocyte count in a dose-dependent manner. Lymphocyte counts returned to normal ranges within 96 h.Almost all multiple myeloma (MM) cases have been demonstrated to be linked to earlier monoclonal gammopathy of undetermined significance (MGUS).

Nevertheless, there are no identified characteristics in the diagnosis of MGUS that have been helpful in differentiating subjects whose cancer may progress to a malignant situation. Regarding malignancy, the role of lymphocyte subsets and cytokines at.